A multimodal cell census and atlas of the mammalian primary motor cortex (MOp or M1) has been achieved as the initial product of the BRAIN Initiative Cell Census Network (BICCN). Coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties, and cellular resolution input-output mapping, integrated through cross-modal computational analysis. These results represent a major milestone in the effort to create a catalog or census of all brain cell types and advance the collective knowledge and understanding of brain cell type organization, and this site provides a guide to the flagship and core companion papers for the work.
This site provides a guide to the flagship and core companion papers for the work.
An extensive genetic toolset was also developed for targeting and fate mapping glutamatergic projection neuron types toward linking their developmental trajectory to their circuit function. Together, the results establish a unified and mechanistic framework of neuronal cell type organization that integrates multi-layered molecular, genetic and spatial information with multi-faceted phenotypic properties.
This includes quick links to BICCN Data Catalog with filtered searches.
By Data Modality
The Flagship paper and Companion papers are listed below with links to the published article DOIs and to visualization tools that support access to data highlighted in those papers.
BRAIN Initiative Cell Census Network (BICCN; Edward M. Callaway, Hong-Wei Dong, Joseph R. Ecker, Mike Hawrylycz, Z. Josh Huang, Ed S. Lein, John Ngai, Pavel Osten, Bing Ren, Andreas S. Tolias, Owen White, Hongkui Zeng, Xiaowei Zhuang)
Zizhen Yao, Hanqing Liu, Fangming Xie, Stephan Fischer,...,Hongkui Zeng, Eran Mukamel.
Trygve Bakken,...,Ed S. Lein.
Hanqing Liu, Jingtian Zhou...,Joseph R. Ecker.
Yang E. Li, Sebastian Preissl,...,Bing Ren.
Meng Zhang,...,Xiaowei Zhuang.
Federico Scala, Dmitry Kobak,...,Philipp Berens, Andreas S. Tolias.
Jim Berg, Staci A. Sorensen, Jonathan T. Ting, Jeremy A. Miller,...,Ed S. Lein.
Zhuzhu Zhang, Jingtian Zhou,...,Joseph R. Ecker, Edward M. Callaway.
Rodrigo Muñoz-Castañeda, Brian Zingg, Katherine S. Matho, Xiaoyin Chen, Quanxin Wang,...,Hong-Wei Dong, Julie A. Harris, Pavel Osten, Z Josh Huang, Giorgio A. Ascoli.
Hanchuan Peng, Peng Xie, Lijuan Liu,...,Hanchuan Peng, Hongkui Zeng.
Katherine Matho,..., Z. Josh Huang.
N N Foster,...,Hong-Wei Dong.
A Sina Booeshaghi,...,Lior Pachter.
Aparna Bhaduri, Carmen Sandoval-Espinosa,...,Arnold Kriegstein.
Ryan Ziffra,...,Tomasz Nowakowski.
Velina Kozareva, Caroline Martin,...,Evan Macosko.
Daniela J. Di Bella,...,Aviv Regev, Paola Arlotta.